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Note that the 6 mm working distance of the Clarity lens will mean that any sample imaged using this lens will most likely have to be mounted in a deep vessel.
Lasers
The DIVE has the following visible solid state lasers for confocal microscopy.
Wavelength |
---|
Colour
Example dyes | |
---|---|
405 | DAPI, Hoechst |
448 | CFP |
488 | GFP, Alexa 488 |
514 | YFP |
561 | mCherry, Alexa 568 |
638 | Cy5, Alexa 647 |
The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm. The fixed and tuneable lines can be used simultaneously.