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Note that the 6 mm working distance of the Clarity lens will mean that any sample imaged using this lens will most likely have to be mounted in a deep vessel.
Lasers
The DIVE has the followingsix visible solid state lasers for confocal microscopy.
Wavelength | Colour | Example dyes |
|---|---|---|
| 405 | Violet | DAPI, Hoechst |
| 448 | Indigo | CFP |
| 488 | Blue | GFP, Alexa 488 |
| 514 | Turquoise | YFP |
| 561 | Yellow | mCherry, Alexa 568 |
| 638 | Red | Cy5, Alexa 647 |
The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm to excite a range of dyes. The fixed and tuneable lines can be used simultaneously.