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The objective lenses in the table below are installed in non-adjacent positions in the standard 6-position nosepiece for objective lenses with M25 threads. Objective lenses are not installed in all positions to prevent damage to adjacent lenses as the objective lens is focused.
| Magnification | Type | NA | Immersion | WD (mm) | Part Number |
|---|---|---|---|---|---|
| 20x | HC PL APO CS2 | 0.75 | Air | 0.62 | 506517 |
| 40x | HC PL APO CS2 | 1.3 | Oil | 0.24 | 506426 |
| 63x | HC PL APO CS2 | 1.4 | Oil | 0.14 | 506350 |
There is also a 10x HC PL APO CS2 0.4 NA (P/N 506424) air lens that can be installed in the standard nosepiece if required.
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The DIVE has six visible solid state lasers for confocal microscopy.
Wavelength | Colour | Example dyes |
|---|---|---|
| 405 | Violet | DAPI, Hoechst |
| 448 | Indigo | CFP |
| 488 | Blue | GFP, Alexa 488 |
| 514 | Turquoise | YFP |
| 561 | Yellow | mCherry, Alexa 568 |
| 638 | Red | Cy5, Alexa 647 |
The infrared laser for multiphoton microscopy is a Coherent Discovery NX with a fixed line at 1040 nm that can be used for red dyes such as tdTomato and mCherry, and a tuneable line that can be adjusted between 660 nm and 1320 nm to excite a range of dyes. The fixed and tuneable lines can be used simultaneously.