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To avoid the possibility of damage to the objective lens during stage initialisation, raise the focus so the objective lens is far away from the stage. There are no focus knobs on the microscope stand itself but there are coarse and fine focus knobs on the STP8000 controller. The inner of the two focus knobs is the coarse focus.

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titleFixed stage microscope

The DM6 is a fixed stage microscope, which means that the stage doesn’t move when the focus is changed. Instead, it’s the objective lens nosepiece that moves up and down. Fixed stage microscopes are used for experiments where the sample must be kept very stable, such as electrophysiological experiments where there may be probes inserted into the specimen that could be moved or damaged if the stage moves up or down. The fixed stage configuration also allows the user to change stage types and raise or lower the stage to accommodate different sized specimens.

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