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The DM6 is a fixed stage microscope, which means that the stage doesn’t move when the focus is changed. Instead, it’s the objective lens nosepiece that moves up and down. Fixed stage microscopes are used for experiments where the sample must be kept very stable, such as electrophysiological experiments where there may be probes inserted into the specimen that could be moved or damaged if the stage moves up or down. The fixed stage configuration also allows the user to change stage types and raise or lower the stage to accommodate different sized specimens. |
Start the software
Double-click the LASX icon on the desktop.
Select one of the configurations from the splash screen drop-down menu.
- machine_MP-Laser_OFF.xlhw - which is for confocal imaging without using the multiphoton laser
- machine_MP-Laser_OFF_climate_ON.xlhw - which is for confocal imaging of live specimens requiring warm incubation
- machine_MP-Laser_ON.xlhw - which is for multiphoton or confocal imaging
- Machine_MP-Laser_ON_climate_ON.xlhw - which is for multiphoton or confocal imaging of live specimens requiring warm incubation
A window will appear asking whether you want to initialise the stage. You should initialise the stage if you need to tile a specimen or capture multiple positions using LASX Navigator. Note that the option to use LASX Navigator will not appear in the software if the stage is not initialised.
Note that the focus should automatically raise the objective lens to the safe Home position when the stage is initialised, but it is wise to manually move the objective lens to a safe position before initialising (see above).
Click Configuration at the top of the LASX user interface. Click the Laser Config button and turn on the lasers you need. The Coherent Discovery laser has a tunable line and a fixed 1040 nm line. MP1 is the fixed 1040 nm line and MP2 is the tunable line. Click MP2, MP1 will automatically come on.