- Make sure the lasers you need for ablation or bleaching are powered on in the software
- If you are going to use the Chameleon multiphoton laser for ablation with infrared light then make sure it is tuned to a suitable wavelength (usually 760 nm).
- If you are going to use the Chameleon, then make sure the correct beam splitting mirror is in place for the invisible laser beam path. For 760 nm this would be the MBS 690+ mirror.
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