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h2. Leica TCS SP5 Confocal Microscope
*Location: MRC Building, first floor, room 1.20B*
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The SP5 is a flexible, fully-featured laser scanning confocal microscope suitable for imaging fixed and live samples. It has an inverted DMI6000B microscope stand capable of fluorescence, bright-field and DIC techniques. The SP5 is a tandem scanner system, which means that it has both a conventional scanner and a fast (8000Hz) resonant scanner for high speed imaging. It is fitted with 5 lasers, producing 8 potential excitation lines, and has 4 PMT detectors and one transmitted light detector. Optical sectioning of samples is achieved using the microscope's integrated focus drive. The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas. FRAP, FRET AB (Acceptor Photobleaching), FRET SE (Sensitised Emission) and Live Data Mode modules are also included with the software. A Pe-Con stage-top incubation system with heat and CO2 control is available for live samples.
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h3. Access & Usage
* [Confocal Booking System (Faces Scheduling System)|http://faces.ccrc.uga.edu]
* [Microscope Booking Rules|Microscope Booking]
* [Microscope Training|LMCB Microscope Training]
h3. Technical Specifications
* [Microscope Information|SP5 Microscope Information]
h3. User Guides
* [Confocal Resolution Calculator|http://lightmicroscopy.lmcb.ucl.ac.uk/Information/LSM_Res.html]
* [Start-up Procedure|SP5 Start-up Procedure]
* [Shut-down Procedure|SP5 Shut-down Procedure]
* [Image Acquisition|SP5 Image Acquisition]
* [How to insert the sample holder|^Insertion.pdf]
* [Image Orientation|^Leica-SP5-Image-Orientation.pdf]
* [Orientation of Stage Movement|^Stage-Orientation.pdf]
* [Using Tile Scan|^LASAF-Tile-Scan-Tool.pdf]
* [Direction of Tile Scanning when seen on the display|^Tile-Scan.pdf]
* [Correcting fluorescence channel mis-registration when using DIC|^SP5TN1.pdf] |
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