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h2. Leica TCS SP5 Confocal Microscope ----- *Location: MRC Building, first floor, room 1.20B* {float:right} !SP5.jpg|border=1! {float} The SP5 is a flexible, fully-featured laser scanning confocal microscope suitable for imaging fixed and live samples. It has an inverted DMI6000B microscope stand capable of fluorescence, bright-field and DIC techniques. The SP5 is a tandem scanner system, which means that it has both a conventional scanner and a fast (8000Hz) resonant scanner for high speed imaging. It is fitted with 5 lasers, producing 8 potential excitation lines, and has 4 PMT detectors and one transmitted light detector. Optical sectioning of samples is achieved using the microscope's integrated focus drive. The software has Tile Scan and Mark & Find modes, which use the microscope's motorised stage to revisit saved positions or build up a 'mosaic' of images of adjacent areas. FRAP, FRET AB (Acceptor Photobleaching), FRET SE (Sensitised Emission) and Live Data Mode modules are also included with the software. A Pe-Con stage-top incubation system with heat and CO2 control is available for live samples. | *Contents* | | {toc:type=list|minLevel=3} | h3. Access & Usage ---- * [Training|LMCB Microscope Training] * [Confocal Booking System (Faces Scheduling System)|http://faces.ccrc.uga.edu] * [Microscope Booking Rules|Microscope Booking] h3. Technical Specifications ---- * [Microscope Information|SP5 Microscope Information] h3. User Guides ---- * [Confocal Resolution Calculator|http://lightmicroscopy.lmcb.ucl.ac.uk/Information/LSM_Res.html] * [Start-up Procedure|SP5 Start-up Procedure] * [Shut-down Procedure|SP5 Shut-down Procedure] * [Image Acquisition|SP5 Image Acquisition] * [How to insert the sample holder|^Insertion.pdf] * [Image Orientation|^Leica-SP5-Image-Orientation.pdf] * [Orientation of Stage Movement|^Stage-Orientation.pdf] * [Using Tile Scan|^LASAF-Tile-Scan-Tool.pdf] * [Direction of Tile Scanning when seen on the display|^Tile-Scan.pdf] * [Correcting fluorescence channel mis-registration when using DIC|^SP5TN1.pdf] |
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