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h2. Leica TCS SP5 Confocal Microscope

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*Location: MRC Building, first floor, room 1.20B*

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The SP5 is a flexible, fully-featured laser scanning confocal microscope  suitable for imaging fixed and live samples. It has an inverted  DMI6000B microscope stand capable of fluorescence, bright-field and DIC  techniques. The SP5 is a tandem scanner system, which means that it has  both a conventional scanner and a fast (8000Hz) resonant scanner for  high speed imaging. It is fitted with 5 lasers, producing 8 potential  excitation lines, and has 4 PMT detectors and one transmitted light  detector. Optical sectioning of samples is achieved using the  microscope's integrated focus drive. The software has Tile Scan and Mark  & Find modes, which use the microscope's motorised stage to revisit  saved positions or build up a 'mosaic' of images of adjacent areas.  FRAP, FRET AB (Acceptor Photobleaching), FRET SE (Sensitised Emission) and Live Data Mode modules are also included with  the software. A Pe-Con stage-top incubation system with heat and CO2  control is available for live samples.

| *Contents* |
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h3. Access & Usage

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* [Training|LMCB Microscope Training]
* [Confocal Booking System (Faces Scheduling System)|http://faces.ccrc.uga.edu]
* [Microscope Booking Rules|Microscope Booking]

h3. Technical Specifications

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* [Microscope Information|SP5 Microscope Information]

h3. User Guides

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* [Confocal Resolution Calculator|http://lightmicroscopy.lmcb.ucl.ac.uk/Information/LSM_Res.html]
* [Start-up Procedure|SP5 Start-up Procedure]
* [Shut-down Procedure|SP5 Shut-down Procedure]
* [Image Acquisition|SP5 Image Acquisition]
* [Using the Hybrid Detectors|LMCB SP5 Hybrid Detectors]
* [How to insert the sample holder|^Insertion.pdf]
* [Image Orientation|^Leica-SP5-Image-Orientation.pdf]
* [Orientation of Stage Movement|^Stage-Orientation.pdf]
* [Using Tile Scan|^LASAF-Tile-Scan-Tool.pdf]
* [Direction of Tile Scanning when seen on the display|^Tile-Scan.pdf]
* [Correcting fluorescence channel mis-registration when using DIC|^SP5TN1.pdf]