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| Standard Objective Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
|---|---|---|---|---|---|
| 10x | Plan Fluor | Air | 0.3 | - | 16 |
| 20x | Plan Fluor | MImm | 0.75 | M | 0.33 - 0.35 |
| 40x | Plan Fluor | Oil | 1.3 | H | 0.2 |
| 60x | Plan Apo | Oil | 1.4 | H | 0.21 |
| Water Dipping Lenses | |||||
| Magnification | Type | Immersion | NA | DIC | WD (mm) |
| 20x | Fluor | Water | 0.5 | M | 2 |
| 40x | Fluor | Water | 0.8 | M | 2 |
| 60x | Fluor | Water | 1.0 | H | 2 |
The letters in column 5 indicate the correct prism for use with the lens | |||||
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Lasers
Argon laser with lines at 458, 476, 488 and 514 nm. Suitable for green fluorescent dyes like EGFP, FITC, Alexa 488 and others. 514 nm line can be used for YFP and related proteins. 458 line can be used for CFP but this is not a very powerful line.
Green HeNe laser (543 nm) for use with orange/red dyes like TRITC, Alexa 532, Alexa 546, Alexa 555, Cy3, DsRed. Can also be used for 'redder' dyes like Texas Red, Alexa 568, Alexa 594, mCherry but with lower excitation efficiency
Red Diode (637 nm) for use with far red dyes like Cy5, Alexa 633, Alexa 647, silicon rhodamine dyes, DRAQ5
Note that there is no 405 nm laser on this machine so you cannot image Hoechst, DAPI or anything else in the blue region of the visible spectrum