Perkin Elmer Ultraview Vox Confocal
Location: Room 1.12, MRC Building
A light tight environmental chamber was fitted on 6th March 2013.
A Yokogawa CSU-X1 spinning disc based system designed for rapid acquisition of 3D (XYZ) and 4D (XYZT) confocal data. The system is built around a Nikon TiE inverted microscope and uses a Hamamatsu C9100-13 EMCCD camera (512 x 512 pixels) to acquire low light level images. A Prior NanoscanZ piezo focus device allows fast collection of image stacks. The TiE microscope is fitted with the Perfect Focus System (PFS), which maintains samples in precise focus indefinitely (only suitable for samples mounted at a glass/aqueous interface). The Vox system also has a Photokinesis Unit (PKU) for spot or ROI illumination in techniques such as FRAP and photoactivation/photoswitching.This page is maintained byLauren McLaughlinand was last updated on the 18th March 2013.
Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z
The following is a procedure I've worked out for setting up the PFS for multi-point acquisition. I used fluorescent HeLa cells grown on Mat-Tek glass-bottomed 35 mm dishes and mounted in a Solent Scientific insert designed for a range of 35 mm dishes but adjusted to hold this one firmly in place. I cannot say for sure that the procedures will work for all surfaces. Nikon only recommend glass of standard #1.5 coverslip thickness (170 µm).
...