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A Yokogawa CSU-X1 spinning disc system designed for rapid acquisition of 3D (XYZ) and 4D (XYZT) confocal data. The system is built around a Nikon TiE inverted microscope and uses a Hamamatsu C9100-13 EMCCD camera (512 x 512 pixels) to acquire low light level images. A Prior NanoscanZ piezo focus device allows fast collection of image stacks. The TiE microscope is fitted with the Perfect Focus System (PFS), which maintains samples in precise focus indefinitely (most suitable for samples mounted at a glass/aqueous interface, but there are some exceptions). The Vox system also has a Photokinesis Unit (PKU) for spot or ROI illumination in techniques such as FRAP and photoactivation/photoswitching.
- Objectives, filters, etcObjective lenses
- Microscope filter sets
- Lasers
- Emission filters
Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z
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