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Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction | Working Distance(mm) |
|---|---|---|---|---|---|
10X | Air | Plan APO Apo | 0.45 | 0.17 | 4 |
20X | Air | Plan APO Apo VC | 0.75 | 0.17 | 1 |
40X | Air | Plan Fluor Apo | 0.75 95 | 0.17 | 0.66 14 |
63X | Oil | Plan Apochromat Apo | 1.4 | 0.17 | 0.13 |
*100X | Oil | VC Plan Fluor Apo | 1.3 4 | 0.15-0.18 17 | 0.28-0.31 13 |
63X | Water | Plan Apochromat Apo | 1.2 | 0.17 | 2 0.27 |
*There is a 100x 1.4NA 3 NA Plan Fluor oil objective that can be used in this space, however it does not readily work with the PSFif the PFS range turns out to be too narrow with the Plan Apo
Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z
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