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Magnification

Immersion Medium

Designation

Numerical Aperture

Coverslip Correction

Working Distance(mm)

10X

Air

Plan Apo

0.45

0.17

4

20X

Air

Plan Apo VC

0.75

0.17

1

40X

Air

Plan Apo

0.95

0.17

0.14

63X

Oil

Plan Apo

1.4

0.17

0.13

* 100X

Oil

VC Plan Apo

1.4

0.17

0.13

63X

Water

Plan Apo

1.2

0.17

0.27

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  • There is also a 100x 1.3 NA Plan Fluor oil objective that can be used if the PFS range turns out to be too narrow

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  • when using the 100x Plan Apo
  • The 60x water lens is useful when imaging beyond about 10 μm in aqueous media. Matching the RI of the immersion media to water helps reduce spherical aberration when imaging far beyond the cover-slip surface
  • There is a choice of three immersion oils:

Oil

Refractive Index

For use with...

Kept in...

Cargille Type HF

1.518 (at 23ºC)

Oil lenses at room temperature

Tray outside environmental chamber

Cargille Type 37

1.518 (at 37°C)

Oil lenses at 37ºC

Tray inside environmental chamber

Zeiss Immersol W

1.334 (at 23ºC)

Water immersion lens only

Tray inside environmental chamber

Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z

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