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Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction | Working Distance(mm) |
|---|---|---|---|---|---|
10X | Air | Plan Apo | 0.45 | 0.17 | 4 |
20X | Air | Plan Apo VC | 0.75 | 0.17 | 1 |
40X | Air | Plan Apo | 0.95 | 0.17 | 0.14 |
63X | Oil | Plan Apo | 1.4 | 0.17 | 0.13 |
* 100X | Oil | VC Plan Apo | 1.4 | 0.17 | 0.13 |
63X | Water | Plan Apo | 1.2 | 0.17 | 0.27 |
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- There is also a 100x 1.3 NA Plan Fluor oil objective that can be used if the PFS range turns out to be too narrow
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- when using the 100x Plan Apo
- The 60x water lens is useful when imaging beyond about 10 μm in aqueous media. Matching the RI of the immersion media to water helps reduce spherical aberration when imaging far beyond the cover-slip surface
- There is a choice of three immersion oils:
Oil | Refractive Index | For use with... | Kept in... |
|---|---|---|---|
Cargille Type HF | 1.518 (at 23ºC) | Oil lenses at room temperature | Tray outside environmental chamber |
Cargille Type 37 | 1.518 (at 37°C) | Oil lenses at 37ºC | Tray inside environmental chamber |
Zeiss Immersol W | 1.334 (at 23ºC) | Water immersion lens only | Tray inside environmental chamber |
Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z
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