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Magnification | Immersion Medium | Designation | Numerical Aperture | Coverslip Correction | Working Distance(mm) |
|---|---|---|---|---|---|
10X | Air | Plan Apo | 0.45 | 0.17 | 4 |
20X | Air | Plan Apo VC | 0.75 | 0.17 | 1 |
40X | Air | Plan Apo | 0.95 | 0.17 | 0.14 |
63X | Oil | Plan Apo | 1.4 | 0.17 | 0.13 |
100X | Oil | VC Plan Apo | 1.4 | 0.17 | 0.13 |
63X | Water | Plan Apo | 1.2 | 0.17 | 0.27 |
There is also a 100x 1.3 NA Plan Fluor oil objective that can be used if the PFS range turns out to be too narrow when using the 100x Plan Apo
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. There is a choice of three immersion oils:
Oil | Refractive Index | For use with... | Kept in... |
|---|---|---|---|
Cargille Type HF | 1.518 (at 23ºC) | Oil lenses at room temperature | Tray outside environmental chamber |
Cargille Type 37 | 1.518 (at 37°C) | Oil lenses at 37ºC | Tray inside environmental chamber |
Zeiss Immersol W | 1.334 (at 23ºC) | Water immersion lens only | Tray inside environmental chamber |
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