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Note that Cargille Type 37 is optimal for use at 37ºC but introduces spherical aberrations when imaging beyond about 10 μm in aqueous media because it is not refractive indexed matched to water. Zeiss Immersol W in combination with the 60x WI lens might be a better choice in this case but its RI is corrected for use at room temperature and won't be correct at 37 degrees.
Filter cubes in the microscope stand
The filter cubes below are for use when viewing your specimen down the microscope eyepieces. The filter turret must be in position 1 (EMPTY) when imaging with the spinning disc.
Position Number | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores |
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1 |
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2 |
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3 |
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4 |
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5 |
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6 |
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Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z
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