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Magnification

Immersion Medium

Designation

Numerical Aperture

Coverslip Correction

Working Distance(mm)

10X

Air

Plan Apo

0.45

0.17

4

20X

Air

CFI Plan Apo Apochromat VC

0.75

0.17

1

40X

Air

CFI Plan Apo Fluor 

0.95 75

0.17

0.14 72

63X 60X

Oil

CFI Plan Apo Apochromat

1.4

0.17

0.13

100X

Oil

CFI Plan Apochromat VC Plan Apo

1.4

0.17

0.13

63X 60X WI

Water

CFI Plan Apo Apochromat

1.2

0.17

0.27

There is also a 100x 1.3 NA Plan Fluor oil objective that can be used if the PFS range turns out to be too narrow when using the 100x Plan Apo.

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