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Position Number | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Example Fluorophores | |||
|---|---|---|---|---|---|---|---|---|
1 | EMPTY | N/A | N/A | N/A | None. For use with bright field or when imaging with the spinning disc | |||
2 | DAPI | D350/50x | 400DCLP |
| D460/50m | DAPI/Hoechst/AMCA | ||
3 | FITC | HQ470/40x | Q495LP |
| HQ525/50m | Endow GFP | ||
4 | TRITC | HQ575/50x | Q610LP |
| HQ640/50m | HcRed1, mCherry | ||
5 | ANALY |
|
|
| N/A | N/A | N/A | DIC analyser (for viewing DIC in eyepieces) |
6 | EMPTY |
|
|
| N/A | N/A | N/A | None. Do not use. |
Setting up the Nikon Perfect Focus System (PFS) for multi-point acquisition in Z
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