Page Comparison

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• Start up and shut down procedure
• Using the Nikon TiE microscope
• Coming Soon - Familiarising yourself with the Vox user interface
• Further Information

Suitable specimens for the Vox

Spinning disc confocal systems perform optimally with adherent cells in culture and other relatively thin specimens. Thicker specimens with a large amount of out-of-focus fluorescence can be difficult to image because of a phenomenon called pinhole crosstalk. Here is a Carl Zeiss technical note explaining pinhole crosstalk.

Microscope stages vary from machine to machine and so do the specimen adapters that they accept. The kinds of specimens the Vox will take is determined by the shape of the cut-out in the NanoscanZ piezo focus. We have two Prior Scientific inserts for this:

• A universal insert that is adjustable and will take slides, Lab-Tek chambered cover-slips, 35 mm² and 60 mm² dishes but that is not suitable for gassing with CO2
• An insert that is compatible with multi-well plate formats and that will accommodate a Solent Scientific CO2 work head that itself is compatible with a range of culture vessels

Objective Lenses

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