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Suitable specimens: All culture vessels must have glass bottoms with the same thickness as a standard #1.5 cover-slip (170 µm). There are inserts for slides, Ibidi µSlides, Lab-Tek chambered cover slips (note - use Lab-Tek I not Lab-Tek II), 35 mm dishes, multi-well plates with square not chamfered corners. If you have any other requirements then you must speak to a member of the light microscopy facility before you start preparing your specimen. Note that CO2 supply is not available for all configurations so check what vessel you need before you start setting up your specimen.
This technical note explains the specimen adapters available for the Vox and how to set up the CO2 supply.
In general spinning disc confocal systems perform optimally with adherent cells in culture and other relatively thin specimens. Thicker specimens with a large amount of out-of-focus fluorescence can be difficult to image because of a phenomenon called pinhole crosstalk.
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