What is super-resolution microscopy?

The resolution of light microscopy is traditionally limited by diffraction to approximately half the wavelength of light, which means any objects smaller than about 250 nm cannot be resolved from one other using light. In recent years super-resolution microscopy techniques have been developed to extend light microscopy beyond this diffraction limit.

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Super-resolution microscopes at the LMCB

The Super-resolution Facility at the LMCB (SuRF) offers the following suite of complementary super-resolution microscope technologies.

• Carl Zeiss Elyra PS.1 - Structured Illumination Microscopy (SIM) and Single molecule Localisation Microscopy (SMLM)
• Leica SP8 STED 3X - Stimulated Emission Depletion (STED) microscopy
• Nikon SoRa - Image Scanning Microscopy (ISM)
• ONI Nanoimager - SMLM, single molecule tracking, smFRET
• 3i Lattice LightSheet – Lattice light sheet microscopy

Using the facility

The achievable resolution and suitability of each technique depends on the specimen and the experimental requirements. If you are interested in using the facility, then please fill in this short web form and the facility staff will arrange a meeting to discuss your requirements.

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