The resolution of light microscopy has is traditionally been limited by diffraction to approximately half the wavelength of light, which means anything any objects smaller than about 250 nm cannot be resolved . Superfrom one other using light. In recent years super-resolution microscopy techniques have been developed to extend light microscopy beyond that barrierthis diffraction limit.
About super-resolution microscopy techniques...
Super-resolution
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Microscopes
The Super-resolution Facility at the LMCB (SuRF) offers a the following suite of complementary super-resolution microscope technologies.
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- Carl Zeiss Elyra PS.1 - Structured Illumination Microscopy (SIM) and Single molecule Localisation Microscopy (SMLM)
- Leica SP8 STED 3X - Stimulated Emission Depletion (STED) microscopy
- Nikon SoRa - Image Scanning Microscopy (ISM)
- ONI Nanoimager - SMLM, single molecule tracking, smFRET
- 3i Lattice LightSheet – Lattice light sheet microscopy
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