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The stage adapters below are fixed to the NanoscanZ using four thumbscrews. It can be fiddly removing and screwing in the thumbscrews so take care not to drop them as they may get caught in the microscope mechanism. The universal adapter on the right is suitable for a variety of specimen formats (e.g. slides, Lab-Tek I, 35 mm dishes) but is not compatible with the CO2 supply. The multi-well plate adapter must be used for procedures requiring CO2 in combination with smaller specimen adapters that fit the CO2 work head.
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| Ibidi µSlide adapter (left) and 35 mm adapter modified for Mat-Tek dishes (right) |
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Connecting the CO2 supply to
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the specimen adapter
The specimen adapters above are all compatible with the CO2 work head (A in the image below). There are two locating pins on each of the adapters that slide into holes in the work head. Make sure the CO2 supply valve (the black and red thing) is on the right (nearest to the humidification bottle and trap). Note that the glass lid of the work head just sits loosely on top of the work head so if you tip it over it will fall off and break.
- Put Slip the CO2 work head on (A) onto the locating pins of your specimen adapter with the red and black valve to the right
- Connect the Luer tube (B) from the CO2the bottle trap to the red and black valve by pressing in the red catch while pushing in the tube (C)
- Make sure there is water in the humidification bottle that's attached to the bottle trap
- Make sure the lids of the trap and humidification bottle are on tight and that all connections are secure
- Make sure any tubing won't get caught by the motorised stage when it moves
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| A. The CO2 work head. B. Luer adapter tube for the gas supply. C. Push the red catch so that the Luer adapter will slide into the work head. |
Supplying CO2 to the work head
Turn on one of the CO2 valves on the wall adjacent to the Vox. Choose 5% or 10% CO2 depending on your medium. The water in the humidifier should start to bubble.
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| Vox CO2 wall valves |