Leica TCS SPE and SPE2
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FAQ
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Training & Booking
Specifications
Objective Lenses
None of the objectives are planar lenses (i.e. they do not have a flat field). Leica recommends zooms ≥1.6 to avoid out-of-focus regions around the edge of the field.
Standard Objective Lenses | |||||
Magnification | Type | Immersion | NA | DIC | Coverslip Correction |
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10x | ACS APO | Air | 0.3 | C | None |
20x | ACS APO | Multi | 0.6 | no | 0 - 0.17 |
40x | ACS APO | Oil | 1.15 | E | 0.17 |
63x | ACS APO | Oil | 1.3 | E | 0.17 |
Water Dipping Lenses | |||||
Magnification | Type | Immersion | NA | DIC | |
10x | HCX APO | Water Dipping | no | None | |
20x | HCX APO | Water Dipping | 0.5 | no | None |
40x | HCX APO | Water Dipping | 0.8 | no | None |
63x | HCX APO | Water Dipping | 0.9 | no | None |
Letters in column 5 indicate the correct DIC prism to use with the lens |
Lasers
Laser | Typical Fluorophores | Dichroic Mirror Position | Notes | |
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405 | DAPI, Hoechst | 405/532 | ||
488 | EGFP, FITC, Alexa 488 | 488/635 | ||
532 | Alexa 532, TRITC, Cy3 | 405/532 | This laser is not brilliant for exciting very red dyes like Alexa 594 and mCherry | |
635 | Cy5, Alexa 633, Alexa 647, Si Rhodamine dyes, DRAQ 5 | 488/635 |
Dichroic Mirror Position: There is a motorised dichroic mirror block that has to switch positions between 405/532 and 488/635 illumination paths, which will limit the speed with which green and red fluorophores (or blue and far red fluorophores) can be imaged. To optimise speed you should set up your multichannel imaging so the blue and red channels are acquired one after another and the green and far red channels are acquired one after another (e.g. blue/red/green/far red). That way the dichroic mirror only has to change position once between channels.
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