Axio Imager
Location: MRC Building, First Floor, Room 1.19.
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Upright fluorescence microscope with phase contrast
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| DescriptionLocation: Room 1.20B, 1st Floor, MRC Building The Zeiss Axio Imager upright microscope is suitable for viewing slide-mounted |
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specimens using phase contrast or fluorescence microscopy. |
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To report problems or request help please email lmcbimage@lmcb.mcbl.ucl.ac.uk.
Objective lenses on Axio Imager
Magnification | Immersion | Designation | NA | Contrast | Coverslip Correction (mm) |
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10X | Air | EC Plan Neofluar | 0.3 | Ph1 | 0.17 |
20X | Air | EC Plan Neofluar | 0.5 | Ph2 | 0.17 |
40X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 |
63X | Oil | Plan Apochromat | 1.4 | Ph3 | 0.17 |
100X | Oil | EC Plan Neofluar | 1.3 | Ph3 | 0.17 |
Fluorescence filter sets on Axio Imager
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Filter Turret
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Turret Position
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Dyes
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Excitation Filter
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Dichroic Mirror
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Emission Filter
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1
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DAPI
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350/50
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400LP
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460/50
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2
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DIC
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N/A
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N/A
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Analyser
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3
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Empty
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N/A
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N/A
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N/A
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4
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GFP
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470/40
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495LP
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525/50
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5
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Texas Red
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560/40
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585LP
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630/75
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6
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Cy5
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620/60
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660LP
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The fluorescence filters on the microscope can be used for conventional fluorophores in the blue, green, red and far red regions of the spectrum. Typical fluorophores would be DAPI, FITC, GFP, TRITC, Cy3, Alexa 647 and Cy5. CFP cannot be imaged with the standard filter sets on this microscope but YFP may be visible in the green channel. CFP and YFP sets do exist to fit this microscope but you must email the light microscopy facility in advance if you want to use them. There are neutral density (ND) filters near the focus on the right of the stand to reduce the intensity of the halogen lamp if it is too bright. The microscope has a QImaging Retiga EXi greyscale CCD camera for high sensitivity fluorescence imaging. The Apple iMac computer currently has Openlab, Volocity and Micro-Manager installed for image acquisition. All icons are in the dock but note that Micro-Manager says 'ImageJ' when you hover the pointer over it. Click on the Technical Resources link for more information about this microscope |
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