Axio Imager Filters

Fluorescence Filters
Filter Set	Excitation Filter	Dichroic Mirror	Emission Filter	Fluorophores
Chroma ET-DAPI	350/50	400LP	460/50	DAPI/Hoechst
DIC (not currently in use)	N/A	N/A	Analyser	DIC
Blank	N/A	N/A	N/A	Transmitted Light
Chroma ET/GFP (FITC/Cy2)	470/40	495LP	525/50	FITC/GFP
Chroma ET/mCherry/Texas Red	560/40	585LP	630/75	Texas Red
Chroma Custom ET Cy5 Longpass	620/60	660LP	665LP	Cy5/Alexa 647
The ET filter sets are very efficient and transmit more light than older sets so specimens may photobleach rather quickly. Use the dial on the lamp to reduce the excitation intensity if bleaching is a problem.

LMCB: Light Microscopy