Axio Imager Filters

Owned by Andrew Vaughan
Last updated: Feb 04, 2016
Fluorescence Filters

Filter Set

Excitation Filter

Dichroic Mirror

Emission Filter

Fluorophores

Chroma ET-DAPI

350/50

400LP

460/50

DAPI/Hoechst

DIC (not currently in use)

N/A

N/A

Analyser

DIC

Blank

N/A

N/A

N/A

Transmitted Light

Chroma ET/GFP (FITC/Cy2)

470/40

495LP

525/50

FITC/GFP

Chroma ET/mCherry/Texas Red

560/40

585LP

630/75

Texas Red

Chroma Custom ET Cy5 Longpass

620/60

660LP

665LP

Cy5/Alexa 647

The ET filter sets are very efficient and transmit more light than older sets so specimens may photobleach rather quickly. Use the dial on the lamp to reduce the excitation intensity if bleaching is a problem.