This microscope is equipped with an an Airyscan 2 unit, a novel 32 channel array detector from Zeiss which can acquire images with up to 8x improvement in Signal to Noise ratio and 1.7x higher resolution than a conventional confocal. It also has 2x multiplex. For more information on how it works, please refer to Zeiss product details
To use the Airyscan, you can either use Smart Setup, selecting Airyscan, and selcting selecting either Resolution, Speed or Sensitivity mode, or by adding Airyscan channels in in "Imaging Setup"
Zoom needs to be set to at least 1.8. The pinhole automatically adjusts to twice the airy unit in order for all the light to go to the scanner instead of being eliminated by the pinhole.
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You can now change the frame size and scan speed.
When setting up your imaging settings, it is advised to image at 16-bit, and to only use 25% of the dynamic range. Using too much of the dynamic range increases processing artefacts when your images are put through Airyscan processing.
Airyscan SR and Airyscan SR-2Y data has to be Airyscan processed to get the final image. Airyscan processing can be done on the same computer, or on Marvin,