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Table of Contents |
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Objective Lenses
Magnification | Immersion |
---|
Type | NA | Contrast Technique | Coverslip Correction (mm) | WD (mm) | ||
---|---|---|---|---|---|---|
10X | Air | N Plan | 0.25 | Ph1 | None | 17.6 |
20X | Air | HCX PL Fluotar LWD | 0.4 | Ph1 | 0 - 2 | 6.9 |
40X | Air | N Plan LWD | 0.55 | Ph2 | 0 - 2 | 3.3 - 1.9 |
10X | Air | C Plan | 0.22 | LMC | None | 7.8 |
20X | Air | N Plan LWD | 0.4 | LMC | 0 - 2 | 3.2 - 1.9 |
40X | Air | N Plan LWD | 0.55 | LMC | 0 - 2 | 3.3 - 1.9 |
Also available... | ||||||
40x | Water | HCX PL APO | 1.1 | N/A | 0.14 - 0.18 | 0.63 |
NA = Numerical Aperture, WD = Working Distance, LWD = Long Working Distance, LMC = Leica Modulation Contrast
Fluorescence Filter Sets
Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
---|---|---|---|---|---|
1 | Leica |
A | BP |
340-380 | 400 |
LP 425 | DAPI, Hoechst, transmitted light |
2 | Leica |
L5 | BP |
480/40 |
505 | BP |
527/ |
30 | FITC, GFP | ||||
3 | Leica N2.1 | BP 515-560 | 580 | LP 590 | Cy3, TRITC |
4 |
Blank
N/A
N/A
N/A
Chroma ET-eCFP | 436/20 | 455 | 480/40 | eCFP |