Leica DMIRB (room 1.19)
Objective Lenses
Magnification |
Immersion |
Designation |
NA |
Contrast |
Coverslip Correction (mm) |
WD (mm) |
---|---|---|---|---|---|---|
10X |
Air |
N Plan |
0.25 |
Ph1 |
None |
17.6 |
20X |
Air |
HCX PL Fluotar LWD |
0.4 |
Ph1 |
0 - 2 |
6.9 |
40X |
Air |
N Plan LWD |
0.55 |
Ph2 |
0 - 2 |
3.3 - 1.9 |
10X |
Air |
C Plan |
0.22 |
LMC |
None |
7.8 |
20X |
Air |
N Plan LWD |
0.4 |
LMC |
0 - 2 |
3.2 - 1.9 |
40X |
Air |
N Plan LWD |
0.55 |
LMC |
0 - 2 |
3.3 - 1.9 |
NA = Numerical Aperture, WD = Working Distance, LWD = Long Working Distance, LMC = Leica Modulation Contrast
Fluorescence Filter Sets
Filter Position |
Filter Set |
Excitation Filter |
Dichroic Mirror |
Emission Filter |
Fluorophores |
---|---|---|---|---|---|
1 |
Leica A4 |
BP 360/40 |
400 |
BP 470/40 |
DAPI, Hoechst |
2 |
Leica GFP |
BP 470/40 |
500 |
BP 525/50 |
FITC, GFP |
3 |
Leica N2.1 |
BP 515-560 |
580 |
LP 590 |
Cy3, TRITC |
4 |
Blank |
N/A |
N/A |
N/A |
Transmitted Light |