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The Elyra is equipped with a levelling stage insert. The There are instructions for levelling the stage are hereon the desktop of the computer. Please do not do it yourself unless you have been trained by facility staff. The levelling feature is important for both SIM and TIRF. However, due to the shape of the insert, certain sample vessels are not suitable. The zeiss sample prep guide has complete instructions, however, there are a few additional points to note below.
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If you wish to use chambered coverslips, we recommend the 2 or 4 chambered ones. the 8-chambered ones are impossible to image with the high NA objectives as the objective has to get too close to the 'arms' of the stage insert as well. This causes huge amounts of frustration to users, and potential damage to the objectives. You will not be able to image the whole surface area of your chambers. The middle 'aisle' of the slide is about the best that we can manage with this sample holder. We also cannot guarantee for TIRF to TIRF may not work with chambered coverslips.