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• Always keep immersion oil bottles in the trays provided. Never put lens tissue or anything else in the trays as it will become contaminated with oil
• Always fully screw the top back onto the immersion oil to prevent big spills if the bottle is knocked over
• Always wear gloves when dispensing immersion oil and remove the gloves as soon as you have applied the oil. This is to stop oil being transferred to keyboards, mice, microscope focus knobs, etc.
• Do not use too much oil. One or two drops from the end of the dropper is almost always sufficient. Excess oil will run down objective lenses or onto the sub-stage condenser and will get inside both the lens and the nosepiece, ruining images.
• Wipe all the lenses you've used after imaging. Use lens tissue to remove oil with a single wipe in one direction. Do not rub back and forth with the tissue as this can scratch the lens. Do not re-use the same tissue but wipe again with another tissue. This stops oil and dirt being transferred. Always wipe up any oil that has run down around the edge of the objective lens to stop oil from getting into the nosepiece.
• Wipe oil and spilled medium from specimen adapters and clean any remaing oil off them with 70% IMS
• Clean up spilled oil immediately by wiping up the excess with absorbent tissue and wiping non-optical components with 70% IMS

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• Contact email for arranging training, reporting problems or requesting help with image acquisition or processing: lmcb-lm-help@ucl.ac.uk
• Departmental Safety Officer's email to report any accidents or incidents: p.topham@ucl.ac.uk
• Members-only mailing list for communicating with other microscope users and sharing information about protocols and procedures: lmcb-lm-users@ucl.ac.uk