Upright fluorescence microscope with phase contrast
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| DescriptionLocation: Room 1.20B, 1st Floor, MRC Building The Zeiss Axio Imager upright microscope is suitable for viewing slide-mounted specimens using phase contrast or fluorescence microscopy. The fluorescence filters on the microscope can be used for conventional fluorophores in the blue, green, red and far red regions of the spectrum. Typical fluorophores would be DAPI, FITC, GFP, TRITC, Cy3, Alexa 647 and Cy5. CFP cannot be imaged with the standard filter sets on this microscope but YFP may be visible in the green channel. CFP and YFP sets do exist to fit this microscope but please you must email the light microscopy facility in advance for if you want to use thesethem. There are neutral density (ND) filters near the focus on the right of the stand to reduce the intensity of the halogen lamp if it is too bright. The microscope has a QImaging Retiga EXi greyscale CCD camera for high sensitivity fluorescence imaging. The Apple iMac computer currently has Openlab, Volocity and Micro-Manager installed for image acquisition. All icons are in the dock but note that Micro-Manager says 'ImageJ' when you hover the pointer over it. Click on the Technical Resources link for more information about this microscope |
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Filter Position | Filter Set | Excitation Filter | Dichroic Mirror | Emission Filter | Fluorophores |
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1 | Chroma ET-DAPI | 350/50 | 400LP | 460/50 | DAPI/Hoechst |
2 | DIC | N/A | N/A | Analyser | DIC |
3 | Empty | N/A | N/A | N/A | Empty |
4 | Chroma ET/GFP (FITC/Cy2) | 470/40 | 495LP | 525/50 | FITC/GFP |
5 | Chroma ET/mCherry/Texas Red | 560/40 | 585LP | 630/75 | Texas Red |
6 | Chroma Custom ET Cy5 Longpass | 620/60 | 660LP | 665LP | Cy5/Alexa 647 |
Note that the ET filter sets are extremely efficient and transmit more light than older filter sets. This goes for the excitation filters as well as the emission filters, so it is possible for specimens to photobleach rather quickly. Please use the intensity dial on the Prior Lumen light source to reduce the excitation intensity if bleaching is a problem.
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