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The instructions below assume that the standard six-position nosepiece is fitted to the DM6 microscope with three standard M25 thread lenses installed: 20x, 40x and 63x. The instructions also assume that the SuperZ Galvo stage is fitted. If an M32 thread lens or an alternative stage is installed, then different procedures will apply.
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The DM6 is a fixed stage microscope, which means that the stage doesn’t move when the focus is changed. Instead, it’s the objective lens nosepiece that moves up and down. Fixed stage microscopes are used for experiments where the sample must be kept very stable, such as electrophysiological experiments where there may be probes inserted into the specimen that could be moved or damaged if the stage moves up or down. The fixed stage configuration also allows the user to change stage types and raise or lower the stage to accommodate different sized specimens. |
Start the software and select a configuration
Double-click the LASX icon on the desktop. Select A splash screen will appear with two drop-down menus for selecting the Configuration and Microscope.
The microscope should always be DM6, but you will need to select one of the following configurations from the splash screen drop-down menu.depending on the hardware features you need:
- machine_MP-Laser_OFF.xlhw - which is for confocal imaging without using the multiphoton laser
- machine_MP-Laser_OFF_climate_ON.xlhw - which is for confocal imaging of live specimens requiring warm incubation
- machine_MP-Laser_ON.xlhw - which is for multiphoton or confocal imaging
- Machine_MP-Laser_ON_climate_ON.xlhw - which is for multiphoton or confocal imaging of live specimens requiring warm incubation
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