Bio-Rad Radiance 2100
Location: MRC Building, Room 1.20B
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Confocal laser scanning microscope
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| This is a Bio-Rad Radiance confocal microscope mounted on a Nikon E800 |
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manual upright microscope stand. |
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are three lasers with six laser lines |
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but there is no 405 nm laser, so you cannot image DAPI or Hoechst. The confocal has |
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for high sensitivity imaging of poorly fluorescent specimens or at low laser power. FRAP |
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and other photo-stimulation experiments are possible |
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Technical Specifications
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SpecificationsObjective LensesObjective Lenses | Magnification | Type | Immersion | NA | DIC | WD (mm) |
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10x | Plan Fluor | Air | 0.3 | - | 16 | 20x | Fluor | Water | 0.5 | M | 2 | 40x | Fluor | Water | 0.8 | M | 2 | 60x | Fluor | Water | 1.0 | H | 2 | The letters in column 5 indicate the correct prism for use with the lens |
LasersLine | Laser | Typical Fluorophores | Notes |
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458 | Argon | CFP | This is not a very powerful laser line | 476 | Argon | - | Could be used as an alternative for green dyes when trying to separate dyes with similar spectra | 488 | Argon | EGFP, FITC, Alexa 488 |
| 514 | Argon | YFP | Could also be used for Citrine, Venus, etc | 543 | GreenHeNe | TRITC, Alexa 532, Alexa 546, Alexa 555, Cy3 | Can also be used for 'redder' dyes like Texas Red, Alexa 568, Alexa 594, mCherry but with lower excitation efficiency | 637 | Red Diode | Cy5, Alexa 633, Alexa 647, Si Rhodamine dyes, DRAQ5 |
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Note that there is no 405 nm laser on this machine so you cannot image Hoechst, DAPI or anything else in the blue region of the visible spectrum Links |
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