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The Elyra is equipped with a levelling stage insert.  The There are instructions for levelling the stage are hereon the desktop of the computer.  Please do not do it yourself unless you have been trained by facility staff.  The levelling feature is important for both SIM and TIRF.  However, due to the shape of the insert, certain sample vessels are not suitable.  The zeiss sample prep guide has complete instructions, however, there are a few additional points to note below. 

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