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Under constructionSample preparation guide from 3i Image processing guide from 3i SlideBook software manual for diSPIM 10x 0.3 NA water lens configuration 40x 0.8 NA water lens configuration 16.7x Special Optics 54-10-12 multi-immersion lens configuration |
The diSPIM (aka 3i Marianas LightSheet) is a dual inverted Selective Plane Illumination Microscope ) is a light sheet microscope in which the sample can be imaged flat on a standard microscope stage. This contrasts with light sheet configurations such as the LightSheet Z.1 in which the sample must be mounted in an imaging chamber, which can require more complex sample preparation steps.
Here is a list of some example research papers in which diSPIM systems have been used for imaging biological specimens.
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