The Super-Resolution Facility (SuRF) at the MRC Laboratory for Molecular Cell Biology (LMCB) houses three commercial super-resolution microscopes covering a range of super-resolution techniques, including Structured Illumination Microscopy (SIM), localization microscopy (PALM/STORM) and Stimulated Emission Depletion (STED) microscopy. Super-resolution microscopy allows the imaging of fluorescently labelled probes at a resolution of just tens of nano-meters, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of photo-switchable fluorophores, high-sensitivity microscopes and single-molecule localisation algorithms make super-resolution imaging rapidly accessible to the wider life sciences research community.
Available super-resolution microscopes:
- Leica TCS SP8 STED 3X (STED)
- Nikon N-STORM (STORM)
- Zeiss ELYRA PS.1 (SIM, PALM/STORM)
Analysis computers for the processing of super-resolution images recorded using the Leica TCS SP8 STED 3X (ORAC) or Zeiss ELYRA PS.1 (ZEN) are located in room 2.19A.
If you would like to use any of the microscopes within the SuRF for your research please apply for facility access by submitting the completed online application form, which can be accessed via the link below:
http://www.ucl.ac.uk/lmcb/Super-Resolution-access-form
More information on super-resolution imaging at UCL can be found here:
http://www.ucl.ac.uk/super-resolution
http://www.ucl.ac.uk/lmcb/research-group/ricardo-henriques-research-group
Some general information on super-resolution imaging can be found here:
http://zeiss-campus.magnet.fsu.edu/articles/superresolution/introduction.html
Presentations from 2015 LMCB Super-Resolution Course:
2015 LMCB Super-Resolution Course - Friday, 25 September 2015 - Sample preparation.pdf
LMCB Super-Resolution Course 2015 - RHenriques Aberrations.pdf