The Zeiss ELYRA PS.1 at the UCL MRC Laboratory of Molecular Cell Biology.
GENERAL INFORMATION
The Zeiss ELYRA PS.1 is capable of both Structured Illumination Microscopy (SIM) and Photo-Activation Localisation Microscopy (PALM). In addition it can be configured for dSTORM, standard TIRF and wide-field microscopy applications. The microscope stage is enclosed in a blacked-out chamber for laser safety reasons and to maintain thermal stability. Super-resolution imaging is recommended and optimal at ambient temperature, but stage-top incubation equipment for live cell imaging applications is available. Multi-colour super-resolution imaging is also possible. The microscope is equipped with four laser lines 405, 488, 561 and 642 nm.
CONTENTS
QUICK START INSTRUCTIONS - Hardware & Software
GETTING STARTED – Putting a Specimen on and Locating it Using the Ocular
GETTING STARTED – SIM Imaging & Image Processing
GETTING STARTED – PALM & 3D PALM Imaging & Image Processing
LINKS
Background information on super-resolution techniques
Zeiss Campus - Structured Illumination Microscopy (SIM)
Zeiss Campus - Photoactivation Localization Microscopy (PALM)
Zeiss Campus - Practical Aspects of PALM Imaging
Quick start instructions poster
Sample preparation
Zeiss ELYRA Sample Preparation Quick Guide 2013.pdf
Zeiss ELYRA PS.1
Zeiss ELYRA - Enter the World of Super-Resolution Brochure 2012.pdf
Super-Resolution Facility (SurF) access application form
SuRF Access Online Application Form