The Super-Resolution Facility (SuRF) at the MRC Laboratory for Molecular Cell Biology (LMCB) houses three commercial super-resolution microscopes covering a range of super-resolution techniques, including Structured Illumination Microscopy (SIM), single molecule localization microscopy (SMLM) and Stimulated Emission Depletion (STED) microscopy. Super-resolution microscopy allows the imaging of fluorescently labelled probes at a resolution of just tens of nano-meters, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of photo-switchable fluorophores, high-sensitivity microscopes and single-molecule localisation algorithms make super-resolution imaging rapidly accessible to the wider life sciences research community.
Available super-resolution microscopes:
- Leica STED (STED)
- Zeiss ELYRA PS.1 (SIM, SMLM)
- ONI Nanoimager (SMLM)
- Nikon SoRa (instant SIM)
Analysis computers for the processing of super-resolution images recorded using the Leica TCS SP8 STED 3X (ORAC) or Zeiss ELYRA PS.1 (ZEN) are located in room 2.19A.
If you would like to use any of the microscopes within the SuRF for your research please apply for facility access by submitting the completed online application form, which can be accessed via the link on the left or here...