MICROSCOPE | ||
|---|---|---|
Nikon N-STORM based on ECLIPSE Ti-E inverted microscope stand |
STORM RESOLUTION | |
|---|---|
XY | ~ 20 - 30 nm |
Z | ~ 50 - 75 nm |
3D STORM axial range | ± 500 nm |
IMAGING MODES |
|---|
STORM (2D or 3D using cylindrical lens) |
Multiparameter widefield with laser or LED illumination (multi-colour, multi-point, time-lapse) |
TIRF |
LIGHT SOURCES | ||
|---|---|---|
Light Source | Type | Wavelengths [nm] |
| Agilent Technologies MLC400 laser combiner | Laser | 405, 488, 561, 647 |
| Lumencor SPECTRA Light Engine® | LED | 395, 440, 470, 508, 555, 640 |
OBJECTIVE LENSES | |||||
|---|---|---|---|---|---|
Objective | Magnification | Immersion Medium | Numerical Aperture | Coverslip Correction [mm] | Working Distance [mm] |
| CFI Plan Fluor 10x | 10x | air | 0.3 | 0.17 | 16 |
| CFI Plan Fluor 40x oil | 40x | oil | 1.3 | 0.17 | 0.2 |
| CFI Plan Apo VC 60x WI | 60x | water | 1.2 | 0.15 – 0.18 | 0.31 – 0.28 |
| CFI Plan Apo VC 60x oil | 60x | oil | 1.4 | 0.17 | 0.13 |
| CFI Apo TIRF 100X Oil | 100x | oil | 1.49 | 0.13 – 0.19 (23°C) 0.14 (37°C) | 0.12 |
FLUORESCENCE FILTER SETS | ||
|---|---|---|
Turret Position | Emission Filter | Example Fluorophores |
| 1 | 49000 | DAPI |
| 2 | 49002 | GFP |
| 3 | 49008 | mCherry |
| 4 | C-S2 | Quadband compatible with all Lumencore lines |
| 5 | C-STO | Quadband compatible with all Agilent laser lines |
| 6 | N-STO | Alexa Fluor 647, Cy5 |