| Excitation Lasers | ||
|---|---|---|
| Wavelength | Type | Fluorophores |
| 405 nm | CW | DAPI, Hoechst, etc |
| White Light Laser | Pulsed | Visible dyes from 470 nm upwards |
| STED Depletion Lasers | ||
| 592 nm | CW | STED on green dyes |
| 660 nm | CW | STED on red dyes |
| 775 nm | Pulsed | STED on far red dyes |
MICROSCOPE: DMI6000, inverted
STED RESOLUTION | |
|---|---|
XY | ~ 50 nm |
Z | same as confocal |
IMAGING MODES:
- Laser scanning confocal imaging (multi-colour, multi-point, live cell)
- STED with 592 nm depletion, up to 3 colours
LIGHT SOURCES | ||
|---|---|---|
Light source | Type | Wavelengths [nm] |
Diode laser | Laser | 405 |
White light laser (WLL) | Laser | 470 - 670, tunable |
Depletion laser | Laser | 592 |
Leica EL6000 | Mercury metal halide bulb | ~ 300 - 700 (peaks at 365, 405, 436, 546, 579) |
A
OBJECTIVE LENSES | |||||
|---|---|---|---|---|---|
Objective | Magnification | Immersion Medium | Numerical Aperture | Coverslip correction [mm] | Working Distance [mm] |
| HCX PL APO CS | 10x | air | 0.4 | 0.17 | 2.2 |
| HC PL FLUOTAR | 10x | air | 0.3 | none | 11 |
| HC PL APO CS2 | 20x | IMM (water, glycerol, oil) | 0.75 | with or without coverglass/correction collar | 0.68 |
| HCX APO LU-V-I | 20x | water | 0.5 | N/A | 35 |
| HC FLUOTAR L VISIR | 25x | water | 0.95 | 0.17 | 24 |
| HC PL APO CS2 | 40x | oil | 1.3 | 0.17 | 0.24 |
| HC PL APO CS2 /1.4 (WD = 0.14 mm) | 63x | oil | 1.4 | 0.17 | 0.14 |
| HC PL APO CS2 (dedicated STED objective) | 100x | oil | 1.4 | 0.17 | 0.13 |
NOTE: There is only space for 6 objective lenses on the objective turret, so if the lens you would like to use is not on there please contact lmcb-lm-help@ucl.ac.uk
FLUORESCENCE FILTER SETS: This microscope uses an Acousto-Optical Beam Splitter (AOBS) with a freely selectable emission detection range rather than fluorescence emission filter cubes.
DETECTORS | |
|---|---|
3x HyD, high sensitivity, 3 operating modes (standard, counting, BrightR) + time-gating | |
3x PMT, one of which for transmitted light imaging | |
A
INCUBATION: The microscope is fully enclosed and equipped with The Cube chamber heater and The Brick CO2 gas mixer.
SOFTWARE | |
|---|---|
Acquistion | LAS X |
Deconvolution | Huygens Professional Version 15.10.1p2 |
A
ADDITIONAL INFORMATION:
- resonant scanner (8000 Hz) for fast imaging
- anti-vibration optical table
- fully enclosed
- autofocus
- motorized XY stage
- Z-galvo