MICROSCOPE: IX83, inverted
IMAGING MODES
- Laser scanning confocal imaging (multi-colour, multi-point, live cell time-lapse, Z-stack)
- FRET
- FRAP
- FLIP
- λ-scan
- uncaging
LIGHT SOURCES
| Light source | Type | Wavelengths [nm] |
| Diode | Laser | 405, 440 |
| Diode | Laser | 559 |
| Diode | Laser | 635 |
| Multi-line Argon ion | Laser | 458, 488. 515 |
| Ablation laser | Laser (pulsed or cw) | 405 |
| U-HGLGPS | Mercury metal halide bulb | ~ 300 - 700 (peaks at 365, 405, 436, 546, 579) |
A
OBJECTIVE LENSES
| Objective | Magnification | Immersion Medium | Numerical Aperture | Coverslip correction [mm] | Working Distance [mm] |
|---|---|---|---|---|---|
| UPLSAPO10X2 | 10x | air | 0.4 | 0.17 | 3.1 |
| UPLSAPO20X | 20x | air | 0.75 | 0.17 | 0.6 |
| UPLFN40X0 | 40x | oil | 1.3 | 0.17 | 0.2 |
| PLAPON60X0SC2 | 60x | oil | 1.4 | 0.17 | 0.12 |
| UPLSAPO60XS | 60x | silicone oil | 1.3 | 0.15 - 0.19 | 0.3 |
| UPLSAPO30XS | 30x | silicone oil | 1.05 | 0.13 - 0.19 | 0.8 |
| The objective lens below is also available for this microscope | |||||
| UPLSAPO60XW | 60x | water | 1.2 | 0.13 - 0.21 | 0.28 |
A
FLUORESCENCE FILTER SETS: Filter sets for imaging a wide range of standard visible and far-red fluorophores are available.
DETECTORS
- 3 channel PMT (Hamamatsu R7862)
- External transmitted light PMT (Hamamatsu R7400)
- Ultra-high sensitivity detector GaAsP with onboard Peltier cooling system (2 channel)
INCUBATION: TokaiHit CO2 stage top incubator including lens heater
SOFTWARE: FV10-ASW 4.2
ADDITIONAL INFORMATION
- anti-vibration optical table
- motorized Z-drift compensator (ZDC)
- motorized XY stage
- Silver coated galvo scanner
- SIM scanner for simultaneous photostimulation and confocal image acquisition