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General Information

Deep Thought is Windows 7 64-bit image analysis PC with dual Intel XEON CPU E5-2620 2 GHz processors (6 cores 12 logical partitions), 160 GB RAM and an AMD Radeon HD 7800 2 GB VRAM graphics card. This computer has almost the maximum amount of RAM possible in Windows 7, which makes it very suitable for memory intensive tasks like image deconvolution. The deconvolution software package Huygens Essential is installed on this machine to make best use of the RAM. There is also a full copy of Carl Zeiss's Zen software installed (both Black and Blue editions) with a module for processing Airyscan data offline.

Huygens Essential

Compatible with Windows Remote Desktop (RDP)

Deep Thought is currently running version 16.05 (built on October 2016) with licences for deconvolving data from widefield, confocal, spinning disk and multiphoton microscopes. The software also has the PSF distiller, which allows you to create point spread functions from sub-resolution bead specimens imaged on your own microscope. Note that the Orac analysis computer has a Huygens sub-licence linked to the STED microscope, which can deconvolve STED and confocal data but not the other modalities listed above. For widefield, spinning disk and multiphoton you will need to use Huygens on Deep Thought.

Zen

Not compatible with Windows Remote Desktop

Deep Thought is currently running Zen 2.1 Sp3 version 14.0.0.201. The copy on this machine is an offline licence linked to the Multiphoton. It has analysis modules relevant to that machine. The most important being probably: FRAP analysis, FCS analysis tools (FCS Basis, FCS Extended, FCS PCH) and Airyscan processing. It is also able to perform linear unmixing of spectral imaging data from Zeiss confocal microscopes.

If you need to process structured illumination imaging (SIM) data from the Elyra microscope the modules for that are only on the Zen analysis computer; not on Deep Thought.

NIS-Elements 5.01

Free image analysis software and browsers:

Fiji (ImageJ)

LAS AF Lite

LAS X

FV10- ASW Viewer

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