Axio Imager
Location: MRC Building, First Floor, Room 1.19.
AxioImager is a Zeiss AxioImager upright microscope ideal for viewing slide-mounted samples using phase contrast or fluorescence microscopy. It has a QImaging QIClick camera designed for taking greyscale images of fluorescent samples with high sensitivity to low light levels. All microscope controls are manual apart from camera image capture, which is through Improvision's Openlab software.
To report problems or request help please email lmcbimage@lmcb.mcbl.ucl.ac.uk.
Objective lenses on Axio Imager
Magnification |
Immersion |
Designation |
NA |
Contrast |
Coverslip Correction (mm) |
|---|---|---|---|---|---|
10X |
Air |
EC Plan Neofluar |
0.3 |
Ph1 |
0.17 |
20X |
Air |
EC Plan Neofluar |
0.5 |
Ph2 |
0.17 |
40X |
Oil |
EC Plan Neofluar |
1.3 |
Ph3 |
0.17 |
63X |
Oil |
Plan Apochromat |
1.4 |
Ph3 |
0.17 |
100X |
Oil |
EC Plan Neofluar |
1.3 |
Ph3 |
0.17 |
Fluorescence filter sets on Axio Imager
Filter Turret |
Turret Position |
Dyes |
Excitation Filter |
Dichroic Mirror |
Emission Filter |
|---|---|---|---|---|---|
1 |
DAPI |
350/50 |
400LP |
460/50 |
|
2 |
DIC |
N/A |
N/A |
Analyser |
|
3 |
Empty |
N/A |
N/A |
N/A |
|
4 |
GFP |
470/40 |
495LP |
525/50 |
|
5 |
Texas Red |
560/40 |
585LP |
630/75 |
|
6 |
Cy5 |
620/60 |
660LP |
665LP |