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  1.  Ensure the microscope has been switched on and initialised, and Zen Black software has been opened for at least 3 hours prior to imaging.
  2.  It is advisable to use the 100x TIRF lens to do PALM/STORM although this is not strictly necessary.  Imaging in TIRF reduces the background and makes PALM reconstruction easier, however TIRF only illuminates objects very close to the coverslip. 
  3.  When ready to image, select laser WF option in the light path menu.  Select 'TV1', which is the Andor CCD for PALM/STORM imaging.  It is advisable to have the 1.6 optovar selected too.
  4.  Set up a track for your most easily located channel and look for the right focus position in Epi mode.
  5.  Then change to TIRF and move the tirf adjustor slider slightly until TIRF is achieved.  This is usually marked by a sudden significant decrease in background fluorescence.
  6.  Reduce image size if required.
  7. Set up a time lapse for ~10000-30000 images.
  8. Start your experiment.
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