GENERAL INFORMATION
The Zeiss ELYRA PS.1 is capable of both Structured Illumination Microscopy (SIM) and Photo-Activation Localisation Microscopy (PALM). In addition it can be configured for dSTORM, standard TIRF and wide-field microscopy applications. The microscope stage is enclosed in a blacked-out chamber for laser safety reasons and to maintain thermal stability. Super-resolution imaging is recommended and optimal at ambient temperature, but stage-top incubation equipment for live cell imaging applications is available. Multi-colour super-resolution imaging is also possible. The microscope is equipped with four laser lines 405, 488, 561 and 642 nm. The microscope is located in room G-09.
LINKS
Background information on super-resolution techniques
Introduction to SIM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/supersim.html
Interactive SIM tutorial: http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/hrsim/indexflash.html
Introduction to PALM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/palm/introduction.html
Practical aspects of PALM: http://zeiss-campus.magnet.fsu.edu/articles/superresolution/palm/practicalaspects.html
Interactive PALM tutorial: http://zeiss-campus.magnet.fsu.edu/tutorials/superresolution/palmbasics/indexflash.html
Sample preparation
Zeiss ELYRA Sample Preparation Quick Guide 2013.pdf
Additional information on the Zeiss ELYRA PS.1
Zeiss ELYRA - Enter the World of Super-Resolution Brochure 2012.pdf
SIM and PALM/STORM image analysis tools
SIMCheck to test the quality of your SIM images: http://www.micron.ox.ac.uk/software/SIMCheck.php