On the panel below the computer monitor, turn on Microscope, Confocal, Lasers and Lamp. Turn on Photomanipulation if you intend to do a photokinetics experiment (FRAP, FRET, FLIM etc)
Then, switch on the computer at the bottom of the 3I tower
If you need temperature control, switch on the OKO Touch chamber by pressing down the 'on' switch for 2 seconds. Set the temperature by touch-tapping the temperature box and using the + and - buttons. You can turn off temperature control by holding down the same power button for 2 seconds and selecting 'OK' when the system asks if you want to switch it off.
If you need CO2, the manual switches are behind the computer. You'll have to go round the back and manually toggle the flow switches into the off or on positions.
The wall mixer dispenses both 5% CO2 and 10% CO2. If the flow controller is perpendicular to the tubing, the flow is off and if it is in-line with the tubing, the flow is on. You can check that the flow is really on by checking if the indicator is floating in the flow regulator.
When the computer starts up, Windows will usually automatically log you into the last user account. At the moment this is standard practice, however we may introduce individual user accounts sometime in the future. Open SlideBook 6 software to begin using the microscope.