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Switching On

  • Turn on the "Main switch" on the keypad
  • Turn on "Systems/PC" on the keypad
  • Wait for computer to boot up and log in to LSM-User account
  • Turn on the "Components" switch on the keypad. 
  • Click the "Start System" button
  • Click the" Acquisition" tab and expand the "Laser" menu. Turn on the lasers you need.  (Some of the lasers might show a red background initially, which means it is not ready to be used.  This will go away after a couple of mintues when the laser is warmed up)

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Locating your Specimen

    • Click the "Locate" tab and expand the "Microscope Control" drop-down menu
    • Select the objective lens you'd like to use - either via the menu in locate', or via the touchscreen controller next to the screen. 
    • Select a suitable fluorescence filter. Only the first three should be used; the rest are special positions fof the confocal and multiphoton
    • Put your specimen on the microscope and open either the transmitted light shutter or the fluorescence shutter depending on which mode you want to use to find your sample
    • Turn on the lamp by clicking its icon and pressing the On button

    • Open the fluorescence shutter to let light through

  • When your specimen is in focus, close the shutter and click the "Acquisition" tab.
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