Bio-Rad Radiance 2100
Location: MRC Building, Room 1.20B
A Bio-Rad Radiance confocal microscope mounted on a Nikon E800 manual microscope stand. The upright microscope is useful for imaging slides or specimens that must be imaged in open chambers. Three lasers with six laser lines: 458, 476, 488, 514 (argon laser); 543 (green HeNe) and 637 (red diode) - Note: There is no 405 laser. Three high sensitivity GaAsP detectors for scanning poorly fluorescent specimens or imaging at low laser powers. Optional water dipping lenses for imaging specimens in open vessels or at long working distances. Optional heated stage adapter (no CO2). FRAP and other photo-stimulation experiments are possible
Advantages of this microscope
Use this microscope for specimens that can only be imaged in open chambers in aqueous media (e.g. tissue, pupae/embryos, fish) or that require a longer working distance (the water dipping lenses have working distances in the order of 2 mm). Live mammalian cells can be imaged if the imaging chamber can be inverted so the cells are upside down. There is a heated stage adapter for warming the specimen but there is no CO2 supply so the chamber must be sealed to prevent pH change. This microscope can also be used to image slides. There is no 405 nm laser, so you cannot image DAPI or Hoechst.