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Currently on the microscope (updated February 2023)

ObjectiveMagnificationImmersion MediumNumerical ApertureCoverslip correction (mm)Working Distance (mm)
CFI Plan Apochromat Lambda D 20x20xAir0.80.170.8
CFI Plan Apo Lambda S 40XC Sil40xSilicone1.250.13-0.210.3
CFI Apo Lambda S 40XC WI40xWater1.250.15-0.190.2-0.16
CFI SR Plan Apo IR 60XC WI60xWater1.270.15-0.190.18-0.16
CFI Apo TIRF 60XC Oil60xOil1.490.13-0.190.16-0.07
CFI SR HP Apo TIRF 200XC Oil100xOil1.490.13-0.190.1-0.16


Available to request (email lmcb-lm-help@ucl.ac.uk)

ObjectiveMagnificationImmersion MediumNumerical ApertureCoverslip Correction (mm)Working Distance (mm)
CFI Plan Apochromat Lambda D 4x4xAir0.20-0.1720
CFI Plan Apochromat Lambda D 10x10xAir0.450.17

4

CFI Apo LWD Lambda S 20XC WI20xWater0.950.11-0.23

0.99-0.9

CFI Apo LWD Lambda S 40XC WI40xWater1.150.15-0.19

0.61-0.59

CFI Plan Apo Lambda S 25XC Sil25xSilicone1.050.11-0.23

0.55

CFI SR HP Plan Apo Lambda S 100XC Sil100xSilicone1.350.15-0.190.31-0.28
CFI Plan Fluor 40x Oil40xOil1.30.170.24
CFI Plan Apochromat Lambda D 60x Oil60xOil1.420.170.15

Filter sets in the CSU-W1 SoRa scan head

  1. DM Wheel (primary dichroic mirror)
  2. Light Path Changer (secondary dichroic mirror)
  3. EM1 Wheel (emission filter wheel for Camera 1)
  4. EM2 Wheel (emission filter wheel for Camera 2)

DM Wheel

Position


1DAPI/FITC/TRITC/Cy5 Quad Band DMMXR00753 - LED - DA/FI/TR/Cy5-B (DAPI/FITC/TRITC/Cy5 Full Multiband Quad) (FF409/493/573/652 - Di02) (Di)Confocal using 405, 488, 561 and 638 nm lasers
2CFP/YFP/mCherry Triple Band DMMXR00723 - LED - CFP/YFP/mCherry-A (CFP/YFP/mCherry - Full Multiband Triple) MXR00537 445/514/594 DM for CSU-W1Confocal using 445, 515 and 561 nm lasers
3350-700 MirrorMXRS1176 Semrock Mirror for CSU-W1For wide-field mode

Light Path Changer

Position
1DM A561LP
2DM A514LP
3VIS-A

EM1 Wheel

Position


1
MXR00753 - LED - DA/FI/TR/Cy5-B (DAPI/FITC/TRITC/Cy5 - Full Multiband Quad) (FF01 - 432/515/595/730) (Em)
2447/60MXRS0705 447/60 BrightLine single-band DAPIDAPI
3472/30MXRS0705 472/30 BrightLine single-band CFPCFP
4525/50MXRS0755 525/50 BrightLine single-band
5542/27MXRS0400 Brightline single-band bandpass YFP emission filterYFP
6600/52MXRS0503 BrightLine single-band bandpass FF01-600/52-25
7641/75MXRS0741 BrightLine single-band bandpass Texas RedTexas Red
8708/75MXRS0612 708/75 BrightLine single-band Cy5Cy5
9809/89

10Full

EM2 Wheel

Position

1
LED - DA/FI/TR/Cy5-B (DAPI/FITC/TRITC/Cy5 - Full Multiband Quad) (FF01 - 432/515/595/730) (Em)
2432/36
3447/60
4472/30
5515/30
6525/50
7542/27
8Empty
9Empty
10Full
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